A facile PCR-RFLP method for genotyping of ITPA rs1127354 and rs7270101 polymorphisms
نویسندگان
چکیده
منابع مشابه
P-241: Association of ITPA Polymorphisms rs1127354 with Infertility
Background: Infertility is a relatively common problem that affects couples worldwide. It is estimated that approximately 1 in 6 couples will experience difficulties in reproducing, defined as a failure to conceive after two years of unprotected sexual intercourse. The molecular and genetic factors underlying the cause of infertility remain largely undiscovered. ITPA is an inosine triphosphatas...
متن کاملThe relationship between ITPA rs1127354 polymorphisms and efficacy of antiviral treatment in Northeast Chinese CHC patients
This prospective study investigated the relationship between 2 inosine triphosphatase (ITPA) polymorphisms (rs7270101 and rs1127354) and the efficacy of ribavirin-based antiviral therapy in hepatitis C virus (HCV)-infected Chinese patients.A total of 906 patients diagnosed with chronic hepatitis C receiving pegylated interferon (PEG-IFN) plus ribavirin combination therapy between January 2011 a...
متن کاملGENOTYPING OF PANDHARPURI BUFFALO FOR k-CASEIN USING PCR -RFLP
The objective of the study was to genotype Pandharpuri buffalo breeds for k-casein type by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A 379-bp k-casein gene fragment was amplified using primers K1 (5’CAC GTC ACC CAC ACC CAC ATT TAT C-3’) and K2 (5’-TAA TTA GCC CAT TTC GCC TTC TCT GT-3’). DNA from 20 Pandharpuri buffaloes were extracted by phenol chloroform me...
متن کاملThe rs1127354 Polymorphism in ITPA Is Associated with Susceptibility to Infertility
OBJECTIVES Infertility is a common human disorder which is defined as the failure to conceive for a period of 12 months without contraception. Many studies have shown that the outcome of fertility could be affected by DNA damage. We attempted to examine the association of two SNPs (rs1127354 and rs7270101) in ITPA, a gene encoding a key factor in the repair system, with susceptibility to infert...
متن کاملInosine triphosphatase allele frequency and association with ribavirin-induced anaemia in Brazilian patients receiving antiviral therapy for chronic hepatitis C
Inosine triphosphatase (ITPA) single nucleotide polymorphisms (SNPs) are strongly associated with protection against ribavirin (RBV)-induced anaemia in European, American and Asian patients; however, there is a paucity of data for Brazilian patients. The aim of this study was to evaluate the ITPA SNP (rs7270101/rs1127354) frequency in healthy and hepatitis C virus (HCV)-infected patients from B...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2018
ISSN: 0887-8013
DOI: 10.1002/jcla.22440